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CD3 (PE)

CD3 (PE)

Cat no: C2253-37G

Supplier: United States Biological
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CD3 is a member of the T cell receptor-associated CD3 complex. The CD3 antigen is expressed on approximately 30% of thymocytes, 70% of blood mononuclear cells and 60% of spleen cells, but < 1% of cells in the bursa and bone marrow.1,2 Applications: � Flow cytometry 1,2 � Immunocytochemistry (acetone-fixed, frozen sections) 2 � Immunosuppression of T cells 2 � Immunoprecipitation 1 Recommended Dilution: Flow Cytometry: (same/less than) 0.5ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2253-37G
Reactivities: Chicken/Bird
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE.
P type: Mab
Isotype: IgG1,k
Purity: Purified
References: 1. Chen, C.-L., L. Ager, G.L. Gartland, and M.D. Cooper. 1986. J. Exp. Med. 164:375-380. 2. Lahti, J.M., C.H. Chen, J.T. Sowder, R.P. Bucy, and M.D. Cooper. 1988. Immunol. Res. 7(4):303.
Additional info: Recognizes chicken CD3. Recognizes a complex of at least three polypeptides of Mr 20-, 19-, and 17kD (two of which are N-glycosylated) on chicken T cells. Also coprecipitates a polypeptide of 90kD from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50- and 40kD.

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