Home  >  Products  >  CD30 (CD30L receptor, D1S166E, Ki-1, KI-1, Ki-1 antigen, Lymphocyte activation antigen CD30, Tumor necrosis factor receptor superfamily member 8)
CD30 (CD30L receptor, D1S166E, Ki-1, KI-1, Ki-1 antigen, Lymphocyte activation antigen CD30, Tumor necrosis factor receptor superfamily member 8)

CD30 (CD30L receptor, D1S166E, Ki-1, KI-1, Ki-1 antigen, Lymphocyte activation antigen CD30, Tumor necrosis factor receptor superfamily member 8)

Cat no: C2382-03K

Supplier: United States Biological
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Human CD30 is a member of the tumor necrosis factor (TNF) receptor family and is expressed on mitogen activated B and T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured CEM human tumor cells were washed and incubated 45 min on ice with 80ul of anti-CD30 antibody at 20ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.39 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2382-03K
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein A affinity chromatography.
References: 1. M.A. Bowen, et al, (1993) J Immunol 151:5896-5906, 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1115-1122, 3. B. Falini, et al, (1995) Blood 85:1-14.
Additional info: Recognizes human CD30. Recognizes the CD30 molecule of about 120kD and blocks binding of recombinant CD153(CD30 L).

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