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CD301 (Macrophage asialoglycoprotein-binding protein1, MGL, DC-ASGPR)

Cat no: C2548-98N


Supplier: United States Biological
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MGL is found on murine mature macrophages in the connective tissue neighboring epithelia of e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. MGL is present on the surface of connective tissue macrophages and their precursor cells in bone marrow. Also, MGL is expressed in macrophage cell lines (e.g. J774-1.6, RAW309Cr.1 and WR19M.1). Expression levels of MGL are increased in mature macrophages. Applications: Suitable for use in Flow cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow cytometry: 1:100-1:200 Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: Hybridization of rat Y3-Ag1.2.3 myeloma cells with spleen cells from immunized rats. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2548-98N
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry
Size: 250ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G chromatography
References: 1. Leenen, P.J.M., et al., (1994),
Additional info: Recognizes mouse CD301, a 38kD cell surface protein that is also known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR). Clone ER-MP23 binds both MGL1 and MGL2 homologues. Clone ER-MP23 has been shown to block the function of mouse CD301.

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