MGL is found on murine mature macrophages in the connective tissue neighboring epithelia of e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. MGL is present on the surface of connective tissue macrophages and their precursor cells in bone marrow. Also, MGL is expressed in macrophage cell lines (e.g. J774-1.6, RAW309Cr.1 and WR19M.1). Expression levels of MGL are increased in mature macrophages.
Applications:
Suitable for use in Flow cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions:
Flow cytometry: 1:100-1:200
Immunohistochemistry: frozen sections
Optimal dilutions to be determined by the researcher.
Hybridoma:
Hybridization of rat Y3-Ag1.2.3 myeloma cells with spleen cells from immunized rats.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.