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CD301 (Macrophage asialoglycoprotein-binding protein1, MGL, DC-ASGPR)

CD301 (Macrophage asialoglycoprotein-binding protein1, MGL, DC-ASGPR)

Cat no: C2548-98N1

Supplier: United States Biological
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MGL is found on murine mature macrophages in the connective tissue neighboring epithelia of e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. MGL is present on the surface of connective tissue macrophages and their precursor cells in bone marrow. Also, MGL is expressed in macrophage cell lines (e.g. J774-1.6, RAW309Cr.1 and WR19M.1). Expression levels of MGL are increased in mature macrophages. The antigen is co expressed with the antigen recognized by monoclonal antibody F0005-51. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and studies on inhibition of biological activity of the mouse macrophage galactose-specific lectin (MGL). Other applications not tested Recommended Dilutions: Flow Cytometry: 1:50 Immunohistochesmitry (Frozen): 1:50 The antigen is glutaraldehyde (0.05%) resistant Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2548-98N1
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
Alternative names: CD301, macrophage asialoglycoprotein-binding protein 1
References: 1. Jansen, A et al; Immunohistochemical characterization of monocytes-macrophages and dendritic cells involved in the initiation of the insulits and beta-cell destruction in NOD mice. Diabetes 1994, 43: 667 2. Rosmalen, J et al; Islet abnormalities associated with an early influx of dendritic cells and macrophages in NOD and NODscid mice. Lab Invest 2000, 80: 769
Additional info: Recognizes the mouse macrophage galactose-specific lectin (MGL), a 38kD single chain surface glycoprotein. Blocking studies demonstrated that the C2548-98N1 is able to block mouse MGL.

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