CD31 is a member of the immunoglobulin superfamily. This heavily glycosylated protein is found in the entire vascular endothelium of adult mice and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. CD31 is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express CD31.
Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:10-1:50 10ul labels 10e6 cells in 100ul.
Immunohistochemistry: frozen
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.