Home  >  Products  >  CD31 (CD31 Antigen, EndoCAM, GPIIA, Platelet Endothelial Cell Adhesion Molecule, Pecam 1, Pecam1, Pecam-1) (Biotin)

CD31 (CD31 Antigen, EndoCAM, GPIIA, Platelet Endothelial Cell Adhesion Molecule, Pecam 1, Pecam1, Pecam-1) (Biotin)

Cat no: C2383-03G3


Supplier: United States Biological
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CD31 (PECAM-1, Platelet gpIIa Molecule), a ~130kD integral membrane glycoprotein, is expressed in platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney, skin biopsies from conditions such as sarcoidosis and lepromatous leprosy and bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 mediates cell-cell adhesion. Valuable iin the study of benign and malignanat vaascular tumors. Staining for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2383-03G3
Reactivities: Rat
Hosts: Mouse
Applications: Flow Cytometry
Conjugates: Biotin
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Flaris, N.A. et al. (1993) Characterisation of microglia and macrophages in the central nervous system of the rat. GLIA 7: 34-40. 2. Williams, K.C. et al. (1996) PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat. J. Neurosci. Res. 45(6):747-57. 3. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells Dev. 18: 1389-98. 4. Ott, I. et al. (2005) Endothelial-like cells expanded from CD34+ blood cells improve left ventricular function after experimental myocardial infarction. FASEB J. 19: 992-4. 5. Fujimoto, K.L. et al. (2007) An elastic, biodegradable cardiac patch induces contractile smooth muscle and improves cardiac remodeling and function in subacute myocardial infarction. J Am Coll Cardiol. 49: 2292-300. 6. Thebault, P. et al. (2010) The C-type lectin-like receptor CLEC-1, expressed by myeloid cells and endothelial cells, is up-regulated by immunoregulatory mediators and moderates T cell activation. J Immunol. 183: 3099-108. 7. Graham, M.J. et al. (1998) In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration. J Pharmacol Exp Ther. 286: 447-58. 8. Haywood, L. et al. (2003) Inflammation and angiogenesis in osteoarthritis. Arthritis Rheum. 48: 2173-7. 9. Kielian, T. and Hickey, W.F. (2010) Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development. Am J Pathol. 157: 647-58. 10. Lochhead, J.J. et al. (2010) Oxidative stress increases blood-brain barrier permeability and induces alterations in occludin during hypoxia-reoxygenation. J Cereb Blood Flow Metab. 30: 1625-36. 11. Nakao, A. et al. (2003) Carbon monoxide inhalation protects rat intestinal grafts from ischemia/reperfusion injury. Am J Pathol. 163: 1587-98. 12. Nakao, A. et al. (2011) Ex vivo carbon monoxide delivery inhibits intimal hyperplasia in arterialized vein grafts. Cardiovasc Res. 89: 457-63. 13. Ohnishi, T. et al. (2007) Comparison of endothelial cell proliferation in normal liver and adipose tissue in B6C3F1 mice, F344 rats, and humans. Toxicol Pathol. 35: 904-9. 14. Schilte, M.N. et al. (2009) Long-term intervention with heparins in a rat model of peritoneal dialysis. Perit Dial Int. 29: 26-35. 15. Seegers, H.C. et al. (2003) Enhancement of angiogenesis by endogenous substance P release and neurokinin-1 receptors during neurogenic inflammation. J Pharmacol Exp Ther. 306: 8-12. 16. Wilson, E. et al. (2007) An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues. J Immunol Methods. 322: 137-42. 17. Willis, C.L. et al. (2010) Protein kinase C activation modulates reversible increase in cortical blood-brain barrier permeability and tight junction protein expression during hypoxia and posthypoxic reoxygenation. J Cereb Blood Flow Metab. 30: 1847-59. 18. Arkudas, A. et al. (2007) Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model. Mol Med. 13: 480-7. 19. Salehi-Had, H. et al. (2011) Utilizing targeted gene therapy with nanoparticles binding alpha v beta 3 for imaging and treating choroidal neovascularization. PLoS One. 6: e18864. 20. Male, D. et al. (1995) An interferon inducible molecule on brain endothelium which controls lymphocyte adhesion mediated by integrins. Immunology 84: 453-460.
Additional info: Recognizes rat CD31, a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leukocytes. Partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.

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