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CD31 (MGC126895, MGC126897)

CD31 (MGC126895, MGC126897)

Cat no: C2383-01X

Supplier: United States Biological
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Human CD31 is an adhesion molecule expressed on platelets, endothelial cells, leukocytes and their bone marrow precursors. CD31 plays a role in homophilic adhesion and heterophilic transendothelial migration (3). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured human Jurkat cells were incubated 45 min on ice with 80ul of anti-CD31 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.62 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2383-01X
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 362-372, 2. P.J. Newman, (1997) J Clin Invest 99: 3-10., 3. K.L. Yong, et al, (1998) Blood 91: 1196-1205.
Additional info: Recognizes human CD31. Reacts with domain 1 of CD31 and blocks homophilic interaction and heterophilic transendothelial migration (1).

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