CD31, a 130kD transmembrane glycoprotein, is a member of Ig superfamily. It is expressed in platelets, monocytes, granulocytes, B cells, certain T-cell subsets and endothelial cells. It exists as a heavily-glycosylated dimer and is a major constituent of endothelial cell intercellular junction. It consists of 6 extracellular Ig-like domains, a transmembrane domain and an ITIM domain within its cytoplasmic domain. Its physiological functions include cell adhesion, transendothelial migration of leukocytes, regulating B-cell activation, vasculogenesis, angiogenesis and integrin activation.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:10-1:25
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Positive Control:
Tonsil
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.