CD312 (EMR2, Epidermal Growth Factor-Like Module-Containing Mucin-Like Receptor 2, Epidermal Growth Factor Seven Transmembrane, EGF-TM7)

EMR2 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulfate, which binds to the fourth EGF-like module of EMR2. \n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications not tested. \n\nRecommended Dilution:\nFlow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul\nImmunohistochemistry (frozen): 1:50-1:200\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2549-36B

Size

500ug

Applications

FC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4. No preservative added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Kwakkenbos. M.J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells. J. Leukoc. Biol.71:854-8622. Stacey. M. et al. (2003) The epidermal growth factor-like domains of the human EMR2 receptor mediate cell attachment through chrondroitin sulphate glycosaminoglycans. Blood.102:2916-2924 3. Kwakkenbos. M.J. et al. (2004) The EGF-TM7 family: a postgenomic view. Immunogenetics. 55:655-666

Additional Info

Recognizes the stalk region of human EMR2.