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CD34 (CD34 Molecule, Hematopoietic Progenitor Cell Antigen CD34) (PE)

CD34 (CD34 Molecule, Hematopoietic Progenitor Cell Antigen CD34) (PE)

Cat no: C2386-05E

Supplier: United States Biological
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CD34 is a membrane glycophosphoprotein expressed by endothelial cells (EC), particularly small vessel EC, and hematopoietic stem and progenitor cells1. Note that the MEC14.7 mAb does not stain as many bone marrow cells as other mouse CD34 mAbs, which is probably due to differences in the glycosolated epitope recognized by each Ab. A sulfated form of CD34 is one of the leukocyte L-selectin counterligands and participates in leukocyte adhesion and homing during the inflammatory process1. CD34 is widely used as a marker to select early hematopoietic stem and progenitor cells in hematopoiesis studies1. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: Excitation 488nm, peak emission 575nm. Immunohistochemistry: frozen sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2386-05E
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 500ul
Form: Supplied as a liquid in sodium azide, BSA.
P type: Mab
Isotype: IgG2a
Purity: Highly Purified
Alternative names: Cluster Designation 34, Endothelial Cell Marker, HPCA1
References: 1. Garlanda, C., et al., Eur. J. Cell Biol. 73:368-377 (1997).
Additional info: Recognizes mouse CD34.

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