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CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1) (Biotin)

Cat no: C2386-01P3


Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2386-01P3
Reactivities: Canine
Hosts: Mouse
Applications: Flow Cytometry
Conjugates: Biotin
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. McSweeney, P. et al. (1998) Characterization of monoclonal antibodies that recognize canine CD34. Blood. 91: 1977-1986.2. Goerner, M. et al. (1999) The use of granulocyte colony-stimulating factor during retroviral transduction on fibronectin fragment CH-296 enhances gene tranfer into hematopoietic repopulating cells in dogs. Blood. 94: 2287-2292.3. Bhattacharya, V. et al. (2000) Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34+ bone marrow cells. Blood. 95: 581-585.4. Goerner, M. et al. (2001) Sustained multilineage gene persistence and expression in dogs transplanted with CD34+ marrow cells transduced by RD114-pseudotype oncoretrovirus vectors. Blood. 98: 2065-2070. 5. Horn, P. et al. (2004) Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol. Blood. 103: 3710-3716.
Additional info: Recognizes canine CD34, a cell surface antigen of approximately 110kD expressed by endothelial cells and hematopoietic stem cells.

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