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CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1)

Cat no: C2386-01P


Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: NS-1/FOX-NY myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2386-01P
Reactivities: Canine
Hosts: Mouse
Applications: Flow Cytometry, Western Blot
Size: 250ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. McSweeney, P. et al. (1998) Characterization of monoclonal antibodies that recognize canine CD34. Blood. 91: 1977-1986.2. Goerner, M. et al. (1999) The use of granulocyte colony-stimulating factor during retroviral transduction on fibronectin fragment CH-296 enhances gene tranfer into hematopoietic repopulating cells in dogs. Blood. 94: 2287-2292.3. Bhattacharya, V. et al. (2000) Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34+ bone marrow cells. Blood. 95: 581-585. 4. Goerner, M. et al. (2001) Sustained multilineage gene persistence and expression in dogs transplanted with CD34+ marrow cells transduced by RD114-pseudotype oncoretrovirus vectors. Blood. 98: 2065-2070.5. Horn, P. et al. (2004) Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol. Blood. 103: 3710-3716.
Additional info: Recognizes canine CD34, a cell surface antigen of approximately 110kD expressed by endothelial cells and hematopoietic stem cells. Reported for use in CD34+ enrichment studies (4,5).

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