CD34 (Cluster of differentiation 34) is a single-pass type I transmembrane glycoprotein which primary functions as a cell to cell adhesion factor. As an adhesion factor, CD34 is expressed during stem/progenitor stage of lymphohematopoietic development and possibly mediates the stem cell attachment to the bone marrow ECM or stromal cells. CD34 is considered to be ideal marker for identifying and quantifying hematopoietic progenitor stem cells. With interaction with CrkL, CD34 becomes a substrate for PKC and CD34 surface expression is associated with activation of PKC.
Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:10,000-1:100,000
Immunohistochemistry: 1:100
Immunocytochemistry: 1: 100-1:250
Flow Cytometry: 1:60
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.