CD35 (C3b/C4b receptor, C3BR, C4BR, Complement receptor type 1, KN)

Cat no: C2387-04F

Supplier: United States Biological

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Description
Additional Information

Human CD35 binds complement components C3b , C4b, and iC3b and is expressed on erythrocytes, B cells, monocytes, granulocytes and folicular dendritic cells and on subsets of thymocytes. CD35 on erythrocytes mediates clearance of immunue complexes. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured U-937 human tumor cells were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD35 antibody at a concentration of 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.70 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	C2387-04F
Reactivities:	Human
Hosts:	Mouse
Applications:	Flow Cytometry, Western Blot
Size:	100ug
Form:	Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride.
P type:	Mab
Isotype:	IgG1
Purity:	Purified by Protein A affinity chromatography.
References:	1. N. Hogg, et al, (1984) Eur J Immunol 14:236-243, 2. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 829-830, 3. C.C. Delibrias, et al, (1994) Eur J Immunol 24:2784-2788, 4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 871-874.
Additional info:	Recognizes human CD35.

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CD35 (C3b/C4b receptor, C3BR, C4BR, Complement receptor type 1, KN)

Cat no: C2387-04F

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