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CD38 (ADP-ribosyl cyclase 1, cADPr hydrolase 1, Cyclic ADP-ribose hydrolase 1, T10)

CD38 (ADP-ribosyl cyclase 1, cADPr hydrolase 1, Cyclic ADP-ribose hydrolase 1, T10)

Cat no: C2390-03D

Supplier: United States Biological
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Human CD38 is a type II transmembrane glycoprotein with bifunctional ectoenzyme activity catalyzing both synthesis and hydrolysis of cyclic ADP ribose (1). CD38 is involved in lymphocyte activation and adhesion to endothelium and has recently been identified as a counter-receptor of CD31 (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured Raji cells per tube were washed and incubated 45 min on ice with 80ul of anti-CD38 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.71 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2390-03D
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 151-157, 2. S. Deaglio, et al, (1998) J Immunol 160:395-402.
Additional info: Recognizes human CD38

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