Home  >  Products  >  CD39 (ATPDase, CD39, DKFZp686D194, DKFZp686I093, Ecto-apyrase, Ecto-ATPase 1, Ecto-ATPDase 1, Ecto-ATP diphosphohydrolase 1, Ectonucleoside triphosphate diphosphohydrolase 1, FLJ40921, FLJ40959, Lymphoid cell activation antigen, NTPDase 1, NTPDase-1)
CD39 (ATPDase, CD39, DKFZp686D194, DKFZp686I093, Ecto-apyrase, Ecto-ATPase 1, Ecto-ATPDase 1, Ecto-ATP diphosphohydrolase 1, Ectonucleoside triphosphate diphosphohydrolase 1, FLJ40921, FLJ40959, Lymphoid cell activation antigen, NTPDase 1, NTPDase-1)

CD39 (ATPDase, CD39, DKFZp686D194, DKFZp686I093, Ecto-apyrase, Ecto-ATPase 1, Ecto-ATPDase 1, Ecto-ATP diphosphohydrolase 1, Ectonucleoside triphosphate diphosphohydrolase 1, FLJ40921, FLJ40959, Lymphoid cell activation antigen, NTPDase 1, NTPDase-1)

Cat no: C2391-01L

Supplier: United States Biological
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Human CD39 is found on most mature B cells, activated NK cells and activated T cells. CD39 has homology to the N- myc family of proteins and has been cloned. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated ~5 min with 20ul of 300ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD39 at 5ug/ml. Cells were washed twice and incubated with reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 15% sub population of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2391-01L
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. J.R. Voland, et al, (1992) Proc Natl Acad Sci USA 89:10425-10429, 2. C.R. Maliszewski, et al, (1994) J Immunol 153:3574-3583, 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 383-385, 4. E. Kaczmarek, et al, (1996) J Biol Chem 271:33116-33122, 5. K. Koziak, S.C. Robson, et al, (2000)J Biol Chem 275(3):2057-2062, 6. Transfusion (May 2006) 46(6):1018-1028, 7. E D Pulte, A J Marcus, et al. (2007) Thrombosis Res 121(3):309-317.
Additional info: Recognizes human CD39.

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