The human gene encoding this enzyme (484 amino acids) has been cloned by Chadwick and Frischauf (1997). The protein shares considerable amino acid identity with the human lymphoid cell activation antigen CD39. The gene is expressed in all tissues but predominantly in the heart in cardiac muscle and capillary endothelial cells. A splice variant has been identified in fetal brain (Yeung et al, 2000). CD39L2 preferentially hydrolyzes nucleotide diphosphates rather than triphospates.
Applications:
Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml detection limit for rhCD39L2 is ~ 2.5ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rhCD39L2 is ~ 5ng/lane under non-reducing and reducing conditions.
Immunoprecipitation: used to immunoprecipitate rhCD39L2 from conditioned media of transfected NS0 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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