Ectonucleoside triphosphate diphosphohydrolase-6 (CD39L2), also known as rhCD39L2 /ENTPD6, is a secreted nucleoside phosphohydrolase, which catalyzes the hydrolysis of extracellular nucleotides, such as nucleoside 5'-diphosphates and nucleoside 5'-triphosphates. It has a broad tissue distribution, with major expression in the heart.
Applications:
Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human CD39L2 is ~1ng/well.
Western Blot: 1-2ug/ml. The detection limit for recombinant human CD39L2 is ~20ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity 5 to 50 fold.
Immunoprecipitation: 25ug/ml to immunoprecipitate recombinant human CD39L2 from conditioned cell culture medium.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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