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CD4 (CD4 Antigen, CD4 Molecule, CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Antigen T4, T Cell Surface Glycoprotein CD4)

CD4 (CD4 Antigen, CD4 Molecule, CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Antigen T4, T Cell Surface Glycoprotein CD4)

Cat no: C2255-02F

Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2255-02F
Reactivities: Bovine
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 2ml
Form: Supplied as a liquid in 0.2M Tris/HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Supernatant
References: 1. Bensaid, A. et al. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4). Vet. Immunol. Immunopathol. 27: 51-54, 2. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle. Vet. Immunol. Immunopathol. 27: 21-27, 3. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334, 4. Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets. Vet. Immunol. Immunopathol. 27: 215-231.
Additional info: Recognizes bovine CD4.

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