CD4, a 55 kD protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance.
Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab Gt xRt
I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRt
Recommended Negative Control:
I1904-82D1: IgG2a, Rat Negative Control
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.