CD4, a 55kD protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+ CD25+), which play a key role in the maintenance of self tolerance.
Applications:
Suitable for use in Flow cytometry. Other applications have not been tested.
Recommended Dilutions:
Flow cytometry: Neat-1:100. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. The Fc region may bind non-specifically to cells expressing low affinity Fc receptors
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.