Home  >  Products  >  CD4 (CD4 Antigen, CD4 Molecule, L3T4, Ly-4, T-cell Surface Glycoprotein CD4, T-cell Differentiation Antigen L3T4, T-cell Surface Antigen T4/Leu-3) (FITC)

CD4 (CD4 Antigen, CD4 Molecule, L3T4, Ly-4, T-cell Surface Glycoprotein CD4, T-cell Differentiation Antigen L3T4, T-cell Surface Antigen T4/Leu-3) (FITC)

Cat no: C2255-04L


Supplier: United States Biological
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CD4, a 55kD protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+ CD25+), which play a key role in the maintenance of self tolerance. Applications: Suitable for use in Flow cytometry. Other applications have not been tested. Recommended Dilutions: Flow cytometry: Neat-1:100. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. The Fc region may bind non-specifically to cells expressing low affinity Fc receptors Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Catalogue number: C2255-04L
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry
Size: 25ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 1% BSA. Labeled with FITC.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G agffinity chromatography.
References: 1. Dialynas, D.P. et al. (1983) Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol.131:2445-2451 2. Wilde, D.B. et al. (1983) Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 131:2178-2183. 3. Dialynas, D.P. et al. (1983) Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 74:29-56.
Additional info: Recognizes mouse CD4. Reported to block CD4+ T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines.

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