CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4, L3T4, LY-4, Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor)

Recognises mouse CD4, a 55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+ CD25+), which play a key role in the maintenance of self tolerance. Clone GK1.5 has been reported to block CD4+ T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines. It may also be used for in vivo and in vitro cell depletion of CD4+ T-cells. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:200 10ul label 1x10e6 cells in 100ul Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with Sspleen cells from immunised Lewis rats. Recommended Secondary Antibodies: I1904-47H IgG, H&L (HRP) Pab GtxRt I1904-47N IgG, H&L, X-Adsorbed (HRP) Pab GtxRt I1904-47T IgG, H&L (HRP) Pab GtxRt I1904-50T1 IgG, H&L (HRP) Pab GtxRt I1904-50U1 IgG H&L, X-adsorbed (HRP) Pab GtxRt I1904-74H IgG, Mouse Ig Adsorbed (HRP) Pab GtxRt I1904-48T IgG, H&L (AP) Pab RbxRt I1904-48Y IgG, H&L, X-Adsorbed (AP) Pab RbxRt I1904-50X1 IgG H&L, X-adsorbed (AP) Pab RbxRt Recommended Negative Control: I1904-82V4 IgG2b, Rat Negative Control I1904-82V3 IgG2b, Rat Negative Control I1904-82V2 IgG2b, Rat Negative Control (Azide Free) I1904-82V1 IgG2b, Rat Negative Control (Biotin) Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.