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CD4, CT (T-cell Surface Glycoprotein CD4, T-cell Surface Antigen T4/Leu-3)

Cat no: C2255-08B


Supplier: United States Biological
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CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Applications: Suitable for use in Flow Cytometry, Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:50 ELISA: 1:1000 Western Blot: 1:50-1:100 Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2255-08B
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human CD4. Species sequence homology: Mouse.

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