CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55kd CD4 protein consists of a 372aa extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120. This soluble CD4 fragment (sCD4) will work in HIV neutralization assays at levels comparable to previously published results. The minor upper band seen in the Coomassie blue-stained gel is likely to be due to the presence of uncleaved signal sequence.
Source:
Recombinant protein corresponding to human CD4 Fragment, fused to His-tag at C-terminal expressed in E.coli.
Molecular Weight:
~27kD
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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