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CD4 (T-Cell Surface Antigen T4/Leu-3, T-Cell Surface Glycoprotein CD4) (APC)

CD4 (T-Cell Surface Antigen T4/Leu-3, T-Cell Surface Glycoprotein CD4) (APC)

Cat no: C2254-92K

Supplier: United States Biological
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Human CD4 is a cell surface glycoprotein of 55kD expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells. CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells. The extracellular portion of CD4 is an array of four Ig-like domains. The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120. Recognizes the domain 1 region of the CD4 molecule. Applications: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: Capture Flow Cytometry: 1:50 - 5x10e5 ficoll prepared human peripheral blood leukocytes were washed and preincubated 5 min with 20ul of 0.3mg/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD4/APC at a 1:50 dilution (5ug/ml). Cells were washed three times, fixed and analyzed by FACS. A 35% sub population stained positive with a mean shift of 2.27 log10 fluorescent units when compared to a Mouse IgG1/APC negative control at a similar concentration. Binding was blocked when cells were pre-incubated 10 min with 20ul of 0.5mg/ml anti-CD4 antibody. Immunohistochemistry: Fixed cells or tissues Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.
Catalogue number: C2254-92K
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry
Size: 120 tests
Form: Supplied as a liquid in 50mM sodium phosphate pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with allophycocyanin (APC).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. D. Healey, et al, (1990) J Exp Med 172: 1233-1242. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 314-330. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 476-478. R. Ko?nig, et al, (1995) J Exp Med 182: 779-787.
Additional info: Recognizes human CD4.

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