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CD4 (T-Cell Surface Antigen T4/Leu-3, T-Cell Surface Glycoprotein CD4) (APC)

CD4 (T-Cell Surface Antigen T4/Leu-3, T-Cell Surface Glycoprotein CD4) (APC)

Cat no: C2254-92M

Supplier: United States Biological
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Human CD4 is a cell surface glycoprotein of 55kD expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells. CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells. The extracellular portion of CD4 is an array of four Ig-like domains. The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: ELISA: Detector Flow Cytometry: 1:50 - 5x10e5 ficoll prepared human PBMC were washed and pre-incubated on ice with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD4/APC at a 1:50 dilution (5ug/ml). Cells were washed three times, fixed and analyzed by FACS. A 52% sub population of cells stained positive with a mean shift of 2.0 log10 fluorescent units when compared to a buffer background. Binding was blocked when cells were pre-incubated 10 min with 20ul of 0.5mg/ml anti-CD4 antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.
Catalogue number: C2254-92M
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 120 tests
Form: Supplied as a liquid in 50mM sodium phosphate pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with allophycocyanin (APC).
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein A affinity chromatography.
References: 1. Characterized by the laboratory of Dr. E.P. Reiber. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 476-478. R. Ko?nig, et al, (1995) J Exp Med 182:779-787.
Additional info: Recognizes human CD4.

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