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CD4 (T-Cell Surface Antigen T4/Leu-3, T-Cell Surface Glycoprotein CD4)

CD4 (T-Cell Surface Antigen T4/Leu-3, T-Cell Surface Glycoprotein CD4)

Cat no: C2254-92J

Supplier: United States Biological
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Human CD4 is a cell surface glycoprotein of 55kD expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells. CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells. The extracellular portion of CD4 is an array of four Ig-like domains. The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120. Recognizes the domain 1 region of the CD4 molecule. Applications: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: Capture Immunohistochemistry: Fixed cells or tissues Flow Cytometry: 5x10e5 cultured HPB-MLT human tumor cells were washed and incubated 45 min on ice with 80ul of anti-CD4 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.84 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2254-92J
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. D. Healey, et al, (1990) J Exp Med 172: 1233-1242. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 314-330. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 476-478. 3. R. Ko?nig, et al, (1995) J Exp Med 182: 779-787.
Additional info: Recognizes the domain 1 region of human CD4. Blocks binding of HIV-1 gp120 protein to CD4 and also blocks HLA Class II Rosette formation.

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