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CD4 (T-cell Surface Glycoprotein CD4, T-cell Surface Antigen T4/Leu-3) (Biotin)

CD4 (T-cell Surface Glycoprotein CD4, T-cell Surface Antigen T4/Leu-3) (Biotin)

Cat no: C2254-93N

Supplier: United States Biological
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CD4 is a 55kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. C2254-93N is useful for in vitro blocking of ligand binding, as well as in vivo CD4+ T cell depletions. Applications: Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Acetone-fixed, frozen Immunohistochemistry: Formalin fixed paraffin embedded Flow Cytometry: (same/less than)0.25ug/10e6 cells in 100ul volume. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2254-93N
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
Conjugates: Biotin
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by immunoaffinity chromatography.
References: 1. Kruisbeek, A.M. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. 2. Nitta, H.,et al., 1997. Cell Vision 4:73. 3. Fan, W. Y., et al., 2001. Exp. Biol. Med. 226: 1045. 4. Muraille, E., et al., 2003. Infect. Immun. 71:2704.
Additional info: Recognizes mouse CD4.

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