CD40 IgG2a Fc, Fusion Protein (Biotin)

Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (1). CD40 muIg fusion protein blocks binding of anti-human CD40 to Raji cells.\n\nMolecular Structure: A soluble fusion protein consisting of the extracellular (193aa) domain of human CD40 fused to murine IgG2a Fc (232aa), with a predicted monomeric molecular weight of 45.6kD.\n\nTransfectant Cell Line: CHO\n\nHuman CD40muIg/Biotin\n\nFunctional Application: CD40-muIg fusion protein blocks binding of anti-human CD40 to Raji human tumor cells.\n\nApplications: \nSuitable for use in ELISA, Flow cytometry. Other applications not tested.\n\nRecommended Dilution\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.\n

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C2392-02J

Size

25ug

Applications

ELISA, FC

Conjugates

Biotin

Form

Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3.

Purity

Purified by affinity chromatography.

References

(1) T.A. Foy, et al, (1996) Annu Rev Immunol 14: 591-617. Purity: Purified by affinity chromatography. \nConcentration: ~0.5mg/ml\n2) M.E. Ozaki, et al, (1997) J Immunol 159: 214-221.\n