CD40 Ligand, soluble, Recombinant, Human (CD40 Ligand, CD40-L, CD40L, CD40LG, hCD40L, sCD40L, CD178, gp39, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Su

Recombinant human soluble CD40 ligand is a 16.3kD protein containing 149aa residues comprising the receptor binding TNF-like domain of CD40L.\n\nCD40 is a member of the TNF receptor family. it is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to ?immunodeficiency with hyper-IgM, a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261aa type II transmembrane protein, which contains a 22aa cytoplasmic domain, a 24aa transmembrane domain and a 215aa extracellular domain. The soluble form of CD40L is an 18kD protein comprising the entire TNF homologous region of CD40L. It is generated in vivo by an intracellular proteolytic processing of the full length CD40L. \n\nCD40 Ligand (CD40L/TRAP, CD154) is a type II membrane protein. It effectuates the helper function of T cells on resting B cells. Recombinant human soluble CD40 Ligand (sCD40L/TRAP) is a 16.3kD protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.\n\nThe CD40 antigen contains four homologous repeating cysteine-rich extracellular domains. It is a single chain glycoprotein. It is known to be a member of the tumor necrosis factor/nerve growth factor superfamily. It shows a significant homology to the Hodgkin

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SPECIFICATIONS

Catalog Number

C2392-30

Size

10ug

Form

Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5. Reconstitute with 5-10mM sodium phosphate, pH 7.5, to a concentration of 0.1-1mg/ml. Do not vortex. Further dilute with PBS, 0.1% BSA or HSA.

Purity

~98% by SDS-PAGE and HPLC. Endotoxin: (same/less than) 0.1ng/ug (1EU/ug)

References

1. Ng-Cashin, J., et al., J. Immunol. 170(8): 4201-4208 (2003). 2. Andreakos, E., et al., Blood 101(3): 983-991 (2003). 3. Grant, E., et al., J. Exp. Med. 196(11): 1461-1471 (2002). 4. Skibinski, G., et al., Immunol. 102: 506-514 (2001).

Alternative Names

CD40 Antigen Ligand, Hyper IgM Syndrome, T-B cell Activating Molecule