CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to ""immunodeficiency with hyper-IgM"", a disease characterized by failure to produce IgG, IgA and IgE. The soluble form of CD40L is an 18kD protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant mouse CD40L is a soluble 16.4kD protein containing 149aa comprising the receptor binding TNF-like domain of CD40L.
Source:
Recombinant protein corresponding to mouse CD40L, expressed in E.coli.
Biological Activity:
Determined by its ability to induce TNF-alpha and MIP-1 alpha production by murine splenocytes. The expected ED50 for this effect is <0.1ug/ml.
Endotoxin:
<0.1ng/ug (1EU/ug)
AA Sequence:
MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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