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CD41a (Biotin)

CD41a (Biotin)

Cat no: C2394-10H

Supplier: United States Biological
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Human CD41 is a member of major platelet transmembrane glycoprotein complex (CD41/CD61) which mediates platelet aggregation and adhesion by serving as the receptor for fibrinogen and von Willebrand factor, fibronectin and vitronectin. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD41a antibody at 5ug/ml. Cells were washed twice and incubated Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. A net 6% sub population of cells stained positive with a mean shift of 2.78 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre-incubated 10 min with 20ul of 0.5mg/ml anti-CD41a antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2394-10H
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Conjugates: Biotin
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with biotin.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing III (A.J. McMichael, et al, eds.) Oxford University Press, Oxford, (1987) p. 744, 2. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 643-648.
Additional info: Recognizes human CD41a-Biotin.

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