GPIb-alpha (platelet glycoprotein Ib-alpha; also CD42b and glycocalicin) is a platelet-derived 145kD member of the leucine-rich glycoprotein family of molecules. Mature human GPIb-alpha is a type I transmembrane protein 610 amino acids (aa) in length. It contains a 489 aa extracellular domain (ECD) (aa 17-505) and a 100 aa cytoplasmic region (aa 527-626). As part of a membrane complex, the GPIb-alpha ECD binds to von Willebrand Factor to impede platelet flow and promote clotting. The human GPIb-alpha ECD is approximately 50% aa identical to dog and mouse GPIb-alpha ECD.
Applications:
Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 25ug/ml use 10ul to label 10e6 cells.
Direct ELISA: 0.5-1.0ug/ml detection limit for rhGPIb-alpha is ~1ng/well.
Western Blot: 1-2ug/ml detection limit for rhGPIb-alpha is ~ 5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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