CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It does not present on resting B cells and erythrocytes. While the 130kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. This antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by this monoclonal antibody is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: (same/less than)1ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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