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CD43 (Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin) (Biotin)

CD43 (Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin) (Biotin)

Cat no: C2397-23D

Supplier: United States Biological
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Human CD43 is a heavily O-glycosylated and sialylated transmembrane protein that is mainly found on T cells, but not on B cells. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Fixed cells or tissues. Flow Cytometry: 5x10e5 cultured human Jurkat-4G cells were incubated 45 min on ice with 80ul of anti-CD43/Biotin at a concentration of 10ug/ml. Cells were washed twice and incubated with Streptavidin/R- PE after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.11 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre-incubated 10 min with 20ul of 0.5mg/ml anti-CD43 antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2397-23D
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Conjugates: Biotin
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with biotin.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. W.P. Stross, et al, (1989) J Clin Pathol 42:953-961, 2. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 1697-1713, 3. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 494-497.
Additional info: Recognizes epitope A of the human CD43 molecule on pMN of about 135kD (1,3). Partially induces apoptosis in hemopoetic progenitor cells and also induces homopoetic aggregation.

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