CD43, a 135kD Type I transmembrane sialoglycoprotein, abundantly expressed on the surface of T-lymphocytes and hematopoietic cells. It is a member of sialomucin family and contains a heavily O-glycosylated extracellular domain, a transmembrane domain and an intracellular cytoplasmic domain. It exists in 2 isoforms; an 115kD form expressed on most T-cells and a 135kD form expressed in resting CD8+ cells. Natural ligands of CD43 are ICAM-1, Galectin-1 and MHC-1 suggesting a complex role of this protein in Leukocyte adhesion mediated by integrins, T-cell activation signaling cascade, anti-apoptotic and pro-apoptotic signaling and anti-adhesive properties based on interaction of each isoform with its ligand. Pathological role of CD43 is indicated in Wiskott-Aldrich syndrome.
Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry (FFPE): 20ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with C2397-25H, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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