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CD44 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)

CD44 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)

Cat no: C2398-05B

Supplier: United States Biological
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CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Interactions between CD44 and HER2 have been linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2398-05B
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied in liquid 10mM HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Supernatant
References: 1. Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196, 2. Cichy, J. and Pure?, E. (2003) J. Cell Biol. 161, 839-843, 3. Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913- 27918, 4. Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407, 5. Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895, 6. Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.
Additional info: Recognizes human CD44. Species crossreactivity: mouse, rat.

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