CD44 is a ubiquitously expressed type 1 transmembrane glycoprotein and proteoglycan, otherwise known as phagocytic glycoprotein 1 (pgp 1), which acts as a receptor for the major extracellular matrix component hyaluronate, and also binds other ligands including collagen and matrix metalloproteinases (MMPs). CD44 is known to play a role in cell-cell interactions, cell adhesion, the activation and homing of lymphocytes, cell migration, hematopoiesis and metastasis, and exists as a number of isoforms generated by the alternative RNA splicing of at least 10 variant exons (CD44v).
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry (Frozen): 50ug/ml
Immunohistochemistry (Paraffin): 20ug/ml. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Use sodium citrate buffer pH 6.0.
Optimal dilutions to be determined by the researcher.
Positive Control:
Breast carcinoma and colon adenocarcinoma
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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