Home  >  Products  >  CD45 (B220, CD45R, GP180, LCA, L-CA, Leukocyte common antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200)
CD45 (B220, CD45R, GP180, LCA, L-CA, Leukocyte common antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200)

CD45 (B220, CD45R, GP180, LCA, L-CA, Leukocyte common antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200)

Cat no: C2399-02J

Supplier: United States Biological
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Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue- specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. CD45 plays an essential role in lymphocyte activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD45 antibody at a concentration of 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.32 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2399-02J
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
References: 1. U. Dianzani, et al, (1992) Eur J Immunol 22:365-371, 2. I.S. Trowbridge & M.L. Thomas, (1994) Annu Rev Immunol 12:85-116, 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 386-399, 4. D. Leitenberg, et al, (1996) J Exp Med 183:249-259.
Additional info: Recognizes human CD45.

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