CD45 is a family of high-molecular weight glycoproteins uniquely expressed on the surface of all leukocytes and their hematopoietic progenitors. The CD45 family consists of multiple members that are all products of a single gene. Structurally, CD45 is composed of one fibronectin extracellular domain, one transmembrane domain, and two cytoplasmic tyrosine phosphatase domains. It is essential for B- and T-cell activation. For both cell-types, CD45 has been shown to interact with kinases including Lyn, src family kinase Lck, and Janus-family kinases. In T cells, CD45 is required for the initiation of receptor signaling by dephosphorylating a negative regulatory tyrosine in the C-terminal tail of Lck. CD45 may also negatively regulate Lck by dephosphorylating the tyrosine in the activation loop, thereby attenuating Lck activity.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (paraffin): 2.5-5ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
Spleen, lymphocytes
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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