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CD45, CT (Protein Tyrosine Phosphatase, Receptor Type, C, B220, CD45, CD45R, GP180, LCA, L-CA, Leukocyte Common Antigen, LY5, T200)

CD45, CT (Protein Tyrosine Phosphatase, Receptor Type, C, B220, CD45, CD45R, GP180, LCA, L-CA, Leukocyte Common Antigen, LY5, T200)

Cat no: C2400-10A

Supplier: United States Biological
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CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:10000 Optimal dilutions to be determined by the researcher. Positive Control: Jurkat lysates Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2400-10A
Reactivities: Human
Hosts: Rabbit
Applications: Flow Cytometry, Western Blot
Size: 100ul
Form: Supplied as a liquid 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Braford, D., et al. (1994). Science. 11:253(5152):1373. 2. Kung, C., et al. (2000). Nature-Medicine. Mar; 6(3):343-345. 3. Virts, E., et al. (1997). Immunology. 34(16-17) 1119-1197.
Additional info: Recognizes human CD45 at and around the C-terminus.

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