CD45 belongs to a family of heavily glycosylated, high molecular weight transmembrane glycoproteins expressed on all cells of haematopoietic origin. Structurally heterogeneous, CD45 consists of a family of eight isoforms ranging from 180,000 to 220,000 MW generated by alternative splicing of three exons differentially expressed on subpopulation of lymphocytes. CD45 protein tyrosine phosphatase activity plays a central role in T-cell activation. CD45 plays an important role in signal transduction inhibition or upregulation of various immunological functions.
Hybridoma: mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD45 protein.
Applications:
Suitable for use in ELISA, Flow Cytometry. Other applications not tested.
Recommended Dilution:
The antibody has been tested by ELISA and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. CD45 is routinely tested on peripheral blood mononuclear cell(PBMC). The histogram is an example of the expected reactivity.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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