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CD45R (B220) (Biotin)

CD45R (B220) (Biotin)

Cat no: C2400-28M

Supplier: United States Biological
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CD45R is an isoform of CD45, also known as B220. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity. Applications: Suitable for use in Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: (same/less than)0.25ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2400-28M
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Conjugates: Biotin
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by immunoaffinity chromatography.
References: 1. Coffman, R.L. 1982. Immunol. Rev. 69:5. 2. George, A., et al., 1994. J. Immunol. 152:1014. 3. Asensi, V., et al., 1989. Immunology 68:204. 4. Domiati-Saad, R., et al., 1993. J. Immunol. 151:5936. 5. Hata, H., et al., 2004. J. Clin. Invest. 114:582. 6. Monteith, C. E. et al., 1996. Can. J. Vet.Res. 60: 193. 7. Shih, F. F., et al., 2006. J. Immunol. 176:3438. 8. Chang, C. L-T., et al., 2007. J. Immunol. 178:6984. 9. Fazilleau, N., et al., 2007. Nature Immunol. 8:753.
Additional info: Recognizes mouse CD45R.

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