This product was clustered as canine CD45R in the First Canine Leukocyte Antigen Workshop (CLAW). It immunoprecipitates an antigen of 200kD from Con-A blast (1). CD45 is expressed on all leukocytes in canine peripheral blood and may be against the CD45R isoforms. CD45R is expressed on subsets of leukocytes (neutrophils are weak) in canine blood of all out bred mongrels and beagles tested.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.