Leukocyte common antigen is expressed exclusively on the surface of hematolymphoid cells and their progenitors, including T and B lymphocytes, granulocytes, monocytes, and macrophages. Mature erythrocytes and megakaryocytes do not express CD45. The CD45 antibody is an important component of the antibody panel used to distinguish hematolymphoid neoplasms from non-hematolymphoid neoplasms, including undifferentiated or anaplastic large cell tumors (malignant lymphoma, melanoma, and carcinoma). In a study of nasophryngeal biopsies, LCA staining was positive for all malignant melanomas and negative for all nasopharyngeal carcinomas. LCA may be used together with cytokeratin antibodies to identify the cell-lineage of anaplastic tumors, demonstrating the ability to identify lymphoma and carcinoma in up to 88% of cases in a single study. Consists of a mixture of three monoclonal antibodies: CD45, CD45RA and CD45RB.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
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