CD45RA (Leukocyte Common Antigen, LCA, Ly-5 T200

Cat no: C2400-41F1

Supplier: United States Biological

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Description
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Leukocyte common antigen is expressed exclusively on the surface of hematolymphoid cells and their progenitors, including T and B lymphocytes, granulocytes, monocytes, and macrophages. Mature erythrocytes and megakaryocytes do not express CD45. The CD45 antibody is an important component of the antibody panel used to distinguish hematolymphoid neoplasms from non-hematolymphoid neoplasms, including undifferentiated or anaplastic large cell tumors (malignant lymphoma, melanoma, and carcinoma). In a study of nasophryngeal biopsies, LCA staining was positive for all malignant melanomas and negative for all nasopharyngeal carcinomas. LCA may be used together with cytokeratin antibodies to identify the cell-lineage of anaplastic tumors, demonstrating the ability to identify lymphoma and carcinoma in up to 88% of cases in a single study. Consists of a mixture of three monoclonal antibodies: CD45, CD45RA and CD45RB. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Paraffin sections. Pre-treatment not required. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	C2400-41F1
Reactivities:	Human
Hosts:	Mouse
Applications:	Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size:	100ug
Form:	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
P type:	Mab
Isotype:	IgG2b
Purity:	Purified by Protein A affinity chromatography.
References:	1. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102: V. Bazil, et al.; Folia Biol (Praha) 35, 289 (1989) 2. Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression: V. Bazil, et al.; Immunogenetics 29, 202 (1989)
Additional info:	Recognizes human CD45RA.

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CD45RA (Leukocyte Common Antigen, LCA, Ly-5 T200

Cat no: C2400-41F1

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