Leukocyte common antigen is expressed exclusively on the surface of hematolymphoid cells and their progenitors, including T and B lymphocytes, granulocytes, monocytes, and macrophages. Mature erythrocytes and megakaryocytes do not express CD45. The CD45 antibody is an important component of the antibody panel used to distinguish hematolymphoid neoplasms from non-hematolymphoid neoplasms, including undifferentiated or anaplastic large cell tumors (malignant lymphoma, melanoma, and carcinoma). In a study of nasophryngeal biopsies, LCA staining was positive for all malignant melanomas and negative for all nasopharyngeal carcinomas. LCA may be used together with cytokeratin antibodies to identify the cell-lineage of anaplastic tumors, demonstrating the ability to identify lymphoma and carcinoma in up to 88% of cases in a single study. Consists of a mixture of three monoclonal antibodies: CD45, CD45RA and CD45RB.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.