CD46, also designated membrane cofactor protein, is a 45-70kD protein with genetic and tissue-specific heterogeneity. It is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to down-regulate the activation of complement on host tissue. It performs this function by serving as a cofactor which binds to C3b and C4b. This binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. It also serves as a receptor for measles virus. Four isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. The isoforms differ in the length. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR).
Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Functional studies. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:50 stains the extracellular domain of CD46.
Western Blot: 1:50 using non-reduced sample treatment and SDS-PAGE detects a band of 45-70kD (ref1,2)
Immunohistochemistry: 1:10
Immunoprecipitation: 1:10; 10e7 cells were lysed and immunoprecipitated with 25ug C2401-02D and 25ug protein G sepharose.
Optimal dilutions to be determined by the researcher.
Recommended Negative Control:
Erthyrocytes
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
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