CD48

CD48, also known as BLAST-1, OX45, and BCM1, is a GPI-linked member of the CD2 subfamily of immunoglobulin superfamily proteins. CD48 is expressed on lymphocytes, monocytes, granulocytes, and mast cells. It functions as a co-stimulatory and adhesion molecule that binds CD2, CD229, and 2B4. CD48 also mediates bacterial phagocytosis by mast cells. Human CD48 shares approximately 50% amino acid sequence identity with mouse and rat CD48, respectively. Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human CD48 (rhCD48; aa 27-220; Accession # NP_001769). Applications: Western Blot, Flow Cytometry, Direct ELISA Recommended Dilution: Western Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect human CD48. Using a colorimetric detection system, the detection limit for rhCD48 is approximately 5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 25ug/mL and add 10ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA : This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human CD48. The detection limit for rhCD48 is approximately 10 ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.