Home  >  Products  >  CD49b (BR, CD49 antigen-like family member B, CD49B, Collagen receptor, GPIa, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2, VLA-2 subunit alpha, VLAA2)
CD49b (BR, CD49 antigen-like family member B, CD49B, Collagen receptor, GPIa, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2, VLA-2 subunit alpha, VLAA2)

CD49b (BR, CD49 antigen-like family member B, CD49B, Collagen receptor, GPIa, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2, VLA-2 subunit alpha, VLAA2)

Cat no: C2404-07X

Supplier: United States Biological
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Human CD49b is an integrin alpha 2 subunit that forms a heterodimer with beta 1 integrin and functions as an adhesion molecule. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Fixed cells or tissues Flow Cytometry: 5x10e5 cultured UM-SCC (squamous cell carcinoma) cells were harvested by trypsinization, washed and preincubated 5 min with 20ul of 250ug/ml human IgG (to block nonspecific binding), after which they were incubated 45 min on ice with 80ul of anti-CD49b antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2404-07X
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by Protein A affinity chromatography.
References: 1. M.L. Tenchini, et al, (1993) Cell Adhesion and Comm 1:55-66, 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1615-1616.
Additional info: Recognizes human CD49b.

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